"Soul Bossa Nova" is a popular instrumental song, composed by and first performed by American impresario, jazz composer, arranger and record producer Quincy Jones. It first appeared on his 1962 Big Band Bossa Nova big band album on Mercury Records. Multi-reed player Rahsaan Roland Kirk played the flute solo. Incomplete personnel on the album liner notes do not specify the prominent brass players.

"Soul Bossa Nova" has endeared itself to producers, musicians and the public. The song appears on the soundtracks to Sidney Lumet's 1964 dramatic film The Pawnbroker, which was scored by Jones, while Woody Allen's 1969 comedy Take the Money and Run features a similar-sounding instrumental composed by Marvin Hamlisch. It was also used by BBC Radio 1 Disc Jockey Alan Freeman as a theme for his afternoon programme that was broadcast on the UK during the 1970s. On 1969, the french composer Nino Ferrer uses the orchestration of the theme for the chorus of his song "les cornichons", based on the title "Big Nick" of James Booker. The theme was used on a long-running Canadian television game show, Definition. Canadian hip hop group Dream Warriors heavily sampled the song for their popular track, "My Definition of a Boombastic Jazz Style", of their debut album And Now the Legacy Begins in 1991. Like Dream Warriors, Canadian Mike Myers grew up watching Definition, and as an homage to his childhood, used the song as the film theme for the Austin Powers film series, starting with Austin Powers: International Man of Mystery (1997). It was used as a theme for the 1998 FIFA World Cup. It was sampled by Ludacris for his Austin Powers-themed 2005 single, "Number One Spot", on his 2004 album The Red Light District, appearing in the videogames Samba de Amigo and Rayman Raving Rabbids TV Party .

The song was also used from 2001 to 2005 as the title song in a German "ethno-comedy" TV-Show named "Was guckst du?" ("What 'ya looking?"), which was based on the british TV-Show "Goodness Gracious Me".

The song was featured in the Pilot episode of "Glee".

The afternoon radio show The Catholic Guy with Lino Rulli, available on Sirius XM, has used this song as its opening theme for three years.